strength lb liquid medium (Beijing Solarbio Science)

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Beijing Solarbio Science strength lb liquid medium
Strength Lb Liquid Medium, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 10930 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lb+liquid+medium/Medium/pm41753614-72-10-20
Average 99 stars, based on 10930 article reviews

strength lb liquid medium - by Bioz Stars, 2026-10

99/100 stars

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Recombinant:

Article Title: Expression, immunogenicity and clinical significance analysis of thyroid‑stimulating hormone receptor fusion proteins.
Article Snippet: Thyroid function is regulated in a substantial manner by thyroid‐stimulating hormone receptor (TSHr), and aberrant alterations in thyroid function are triggered by the interaction of TSHr with its antibodies, thyroid‐stimulating hormone receptor antibodies (Trab).. The expression, immunogenicity and clinical significance of fusion proteins comprising different structural domains of TSHr were investigated.. Fusion proteins containing several human TSHr (hTSHr) structural domains were created.

Article Title: Expression, immunogenicity and clinical significance analysis of thyroid-stimulating hormone receptor fusion proteins
Article Snippet: They were confirmed and identified using 2% agarose gel electrophoresis (visualization method, green fluorescent nucleic acid dye; cat. no. G8140; Beijing Solarbio Science & Technology Co., Ltd.) and imaging (BluSight Pro; GD50502; Monad Biotech Co., Ltd.). .. Positive colonies were inoculated into LB liquid medium (Beijing Solarbio Science & Technology Co., Ltd.) with 100 μg/ml ampicillin (Beijing Solarbio Science & Technology Co., Ltd.) and shaken overnight (37°C; 220 rpm), and plasmids were extracted from recombinant TOP10-hTSHR cells using a plasmid mini-prep kit (TIANprep Mini Plasmid Kit; cat. no. DP103; Tiangen Biotech Co., Ltd.) according to the manufacturer's instructions. .. After collecting the bacteria, using the plasmid mini-prep kit, Buffer P1 solution, Buffer P2 solution and Buffer P3 solution containing RNaseA were added sequentially, and the supernatant was moved into the adsorption column CP3 and centrifuged for 2 min at room temperature at 13,400 × g, and the elution buffer EB was added dropwise to the middle part of the adsorption membrane, and the recombinant plasmid solution containing the target gene was collected into the centrifuge tube after centrifugation for 2 min at room temperature at 13,400 × g. Using a 3730×l DNA Analyzer (cat. no. A41046; Thermo Fisher Scientific Inc.), the extracted recombinant plasmid was used as a template, and the primers used were: Forward, TTCCTCGACGCTAACCTG and reverse, TAGTTATTGCTCAGCGGTGG.

Article Title: Genome-Wide Identification and Functional Divergence of the Chloride Channel (CLC) Gene Family in Autotetraploid Alfalfa ( Medicago sativa L.)
Article Snippet: .. Agrobacterium tumefaciens harboring the recombinant plasmids were cultured in LB liquid medium supplemented with kanamycin (50 mg/L) and rifampicin (25 mg/L) (Solarbio, Beijing, China) at 28 °C with shaking at 200 rpm for 24 h, after which the bacterial cells were collected. ..

Plasmid Preparation:

Bacteria:

Article Title: V-ATPase B mediates Bt Cry1Ac binding and toxicity in Grapholita molesta.
Article Snippet: Grapholita molesta is a worldwide pest.. Cry1Ac is a significant alternative Bacillus thuringiensis (Bt) protein that exhibits substantial toxicity towards Lepidoptera insects.. This investigation employed ligand blot, SDS-PAGE and liquid chromatography-tandem mass spectrometry (LC-MS/MS) techniques to identify the proteins potentially binding Cry1Ac on the peritrophic membrane (PM) of G. molesta.

Adsorption:

Membrane:

Centrifugation:

Incubation:

Article Title: Generalizability of Enzyme‐Based Isolation Approach for Extracellular Vesicles From Traditional Medicinal Plants
Article Snippet: .. To examine bacterial growth, the reaction solution (0.4 mL) was aspirated, spread onto Luria–Bertani (LB) agar plates, and incubated at 37°C for 24 h. To detect fungal contamination, the reaction solution (1 mL) was mixed with LB liquid medium (5 mL) and incubated overnight at 37°C, followed by glycogen staining on a slide (100 μL) with periodic acid‐Schiff (PAS) staining (G1366, Solarbio, China) according to the manufacturer's instructions. ..

Article Title: The nonstructural protein 13 of porcine deltacoronavirus coordinates ATP-driven duplex unwinding and ATP-independent strand annealing for nucleic acid remodeling
Article Snippet: .. Monoclonal colonies were inoculated into 5 mL LB liquid medium containing kanamycin (Solarbio Life Science, Beijing, China) and incubated at 220 r/min in a constant temperature shaker at 37 °C for 12 h, then the culture was inoculated into LB liquid medium at a ratio of 1:100 to expand the culture, and induced with 0.1 mM IPTG when the optical density reached 0.6. ..

Article Title: The nonstructural protein 13 of porcine deltacoronavirus coordinates ATP-driven duplex unwinding and ATP-independent strand annealing for nucleic acid remodeling.
Article Snippet: .. Monoclonal colonies were inoculated into 5 mL LB liquid medium containing kanamycin (Solarbio Life Science, Beijing, China) and incubated at 220 r/min in a constant temperature shaker at 37 °C for 12 h, then the culture was inoculated into LB liquid medium at a ratio of 1:100 to expand the culture, and induced with 0.1 mM IPTG when the optical density reached 0.6. ..

Staining:

Cell Culture:

Article Title: Vitamin E Enhances Immune Function and the Intestinal Histological Structure by Regulating the Nodal -Mediated Signaling Pathway: A Case Study on the Sea Cucumber Apostichopus japonicus
Article Snippet: Upon obtaining the desired plasmid, it was transferred into E. coli HT115 competent cells (AngYubio, Shanghai, China). .. Single colonies were inoculated into 50 mL of LB liquid medium containing 100 mg/L ampicillin and 12.5 mg/L tetracycline (Solarbio, Beijing, China) and cultured until the optical density (OD) reached 0.5–0.6, followed by sequencing (TianGen, Beijing, China). .. Isopropyl β-D-1-thiogalactopyranoside (IPTG) (Solarbio, Beijing, China) was added to a final concentration of 0.5 mM, and expression was induced at 37 °C for 4 h to obtain the experimental culture [ ].

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